Early work which identified DNA as the primary agent of genetic material.
The structure of DNA and RNA. The double helix.
DNA Replication - Introduction to Prokaryotic replication.
Growth in bacterial populations
Extrachromosomal elements, plasmids, selectable markers
Transcriptional Regulation: the lac operon, cont., DNA footprinting
Transcriptional Regulation: Transcription termination, the trp operon
Polymerase Chain Reaction (PCR)
Prokaryotic Expression Vectors
Protein Sequencing, Peptide Mapping, Synthetic Genes
Protein Purification: Assays, Specific Activity, Initial Fractionation
Protein Purification: Column Chromatography - Ion exchange; Dialysis and Concentration
Protein Purification: Running the Experiment, Resolving Peaks
SELEX (Selective Evolution of Ligands by Exponential Enrichment)
Protein-Protein Recognition Probed Using a Yeast Transcriptional Activator System
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